Journal of Molecular Biology (2018). Volume 430, Issue 10, 11 May 2018, Pages 1495-1509. Available online 4 April 2018. https://doi.org/10.1016/j.jmb.2018.03.027
Pulmonary surfactant protein D (SP-D) is a glycoprotein from the collectin family that is a component of the lung surfactant system. It exhibits host defense and immune regulatory functions in addition to contributing to the homeostasis of the surfactant pool within the alveolar airspaces. It is known that the SP-D monomer forms trimers, which further associate into higher-order oligomers. However, the pathway and the interactions involved in the assembly of SP-D oligomers are not clearly understood. In the current study, a recombinant form of full-length human SP-D (rhSP-D) has been qualitatively and quantitatively studied by atomic force microscopy (AFM) and electrophoresis, with the aim to understand the conformational diversity and the determinants defining the oligomerization of the protein. The rhSP-D preparation studied is a mixture of trimers, hexamers, dodecamers and higher-order oligomeric species, with dodecamers accounting for more than 50% of the protein by mass. Similar structures were also found in hSP-D obtained from proteinosis patients, with the largest fuzzy-ball-like oligomers being more abundant in these samples. The proportion of dodecamer is increased under acidic conditions, accompanied by a conformational change into more compact configurations. Two hexamers appear to be the minimal necessary unit for dodecamer formation, with stabilization of the dodecamer occurring via non-covalent, ionic, and hydrophobic interactions between the individual N-terminal domains and the proximal area of the SP-D collagen stems.
Feb (2018). A. Martin-Gonzalez participates at FotCiencia contest.
Feb (2018). J. Madariaga-Marcos talk at the Mechanobiology Seminars at CNIC, Madrid.
Feb (2018). Dr Gijs J. L. Wuite visits the group and gives a talk at CNB entitled “Frontiers in Single Molecule Manipulation and Imaging of DNA-protein transactions”.
Feb (2018). Several people from the group participates at the events for the February11 celebration of the “International Day of Women and Girls in Science” .
Feb (2018). New paper accepted at Nanoscale on lateral magnetic tweezers combined with TIRF microscopy.
Feb (2018). J. Madariaga-Marcos talk and poster at Biophysical Society Meeting, San Francisco.
Feb (2018). F. Moreno-Herrero participation at Biophysical Society Meeting, San Francisco.