The 2023 edition of the Macromolecular
Structures Department Workshop at the CNB-CSIC will focus on Advanced Biophysical Approaches to foster our knowledge on protein spectrometry and sequencing, nucleic acids nanostructures, mechanisms of molecular motors, nanopores, and mechanobiology.


Mass Photometry

Protein Sequencing

Optical & Magnetic Tweetzers

Atomic Force Microscopy

Fluorescence based methods


9:20 – 9:30 Welcome
F. Moreno-Herrero. Main Organizer (CNB-CSIC)

Session 1: Advanced biophysical approaches based on microscopy, optical and magnetic tweezers

Session Chair: Fernando Moreno-Herrero (CNB-CSIC, Madrid)

9:30 – 10:15 Keynote TALK

Unravelling biomolecular structure, interactions and dynamics with mass photometry

Philipp Kukura (University of Oxford, UK)

10:15 – 10:35

Unveiling the contribution of  lncRNA NIHCOLE to the repair of DNA breaks using Magnetic Tweezers and Fluorescence-correlated Optical Trapping
Sara de Bragança (CNB-CSIC, Madrid)

10:35 – 10:55

Comparative analysis of force generation by dynamin isoforms during membrane remodelling
Borja Ibarra (IMDEA-Nanociencia, Madrid, Spain)

10:55 – 11:15

Exploring DNA repair at the single-molecule level
Silvia Hormeño (CNB-CSIC, Madrid)

11.15 – 12.00 Coffee Break (sponsored by LumicksTM ) + Poster Sesion

Session 2: Advanced biophysical approaches based on nanopores

Session Chair: Sara de Bragança (CNB-CSIC, Madrid)

12:00 – 12:45 Keynote TALK

The timing of life at the nanoscale

Sonja Schmid (Wageningen University & Research, The Netherlands)

12:45 – 13:05

DNA nanotechnology to engineer advanced nanomaterials for biomedical applications
Silvia Hernandez-Ainsa (Institute of Nanoscience and Materials of Aragon, CSIC-University of Zaragoza, Zaragoza)

13:05 – 13:25

Analysis of single protein molecules with nanopores
David Rodriguez-Larrea (Instituto Biofisika, Bilbao)

13:25 – 13:45

Getting a grip on dynamic single molecules with correlative optical tweezers
Emma Verver (LUMICKS TM, The Netherlands)

13:45 – 15:00 Lunch + Posters Sesion

Session 3: Advanced biophysical approaches based on AFM

Session Chair: Mikel Marin Baquero (CNB-CSIC, Madrid)

15:00 – 15:45 Keynote TALK

Decoding mechanical fingerprints of cellular components in pathological conditions: a multiscale perspective

Andra Dumitru (CNIC, Spain)

15:45 – 16:05

Physical Virology with atomic force and fluorescence microscopies: exploring the biophysics of individual virus particles
Pedro J. de Pablo (UAM, Madrid)

16:05 – 16:25

How much force is needed to kill a single bacterium?
Cristina Flors (IMDEA-Nanociencia, Madrid)

16:25 – 16:45

Structural analysis of single-stranded RNA molecules using Atomic Force Microscopy
Eva M. Martin (CNB-CSIC, Madrid)

16:45 – 17:05

Nanorheology and Nanoindentation Revealed a Softening and an Increased Viscous Fluidity of Adherent Mammalian Cells upon Increasing the Frequency
Victor G. Gisbert (ICMM, Madrid)

17:05 – 17:25

Bridging the scales in DNA flexibility from nucleotides to single-molecule techniques via molecular simulations
Salvatore Assenza (UAM, Madrid)

17:25 Closing and farewell

If you are a SEBBM member and would like to attend on site.

Please send an email to and we will give you information about travel grants