Fernando Moreno-Herrero | Biophysical Lab

We are interested in studying dsDNA break repair and Chromosome organisation at the single molecule level using Atomic Force Microscopy, Magnetic Tweezers, Fluorescence, and standard biochemical techniques.

Welcome to the Moreno-Herrero Lab

The main interest of my group is to answer key questions in DNA-break repair, replication and Chromosome organisation using novel approaches based on single-molecule techniques. To do this, we develop our own instrumentation based on Atomic Force Microscopy imaging, single-molecule manipulation techniques such as Magnetic Tweezers combined with fluorescence, and establish strategic collaborations with research groups specialized on different biological systems. We are also interested in studying the mechanical properties of nucleic acids and their role in protein interaction. We investigate this from an experimental perspective using our single-molecule tools but also by all-atom molecular dynamics simulations.

We work at the National Center of Biotechnology (CNB), a research center part of the Spanish National Research Council (CSIC). The CNB is the largest CSIC institute with over 600 people working in a multidisciplinary environment that combines the latest technology in molecular biology, and structural and functional biology.

Check out our Openings section for opportunities to join the group to do the Master project, PhD or Postdoctoral research.

MORE ABOUT OUR LAB

MORE ABOUT ME

MD simulations of Nucleic Acids Mechanical Properties

Mechanical Properties of nucleic acids Mechanical Properties

High-resolution AFM imaging of nucleic acids Mechanical Properties

DNA-end processing DNA Repair

SMC proteins DNA Organization

Type III partition systems DNA Organization

ParABS partition systems DNA Organization

Rolling Circle Replication of plasmids DNA Replication

Replication initiation proteins DNA Replication

Magnetic Tweezers Techniques

Combined MT-TIRF Techniques

BioLab Techniques

Atomic Force Microscopy Techniques

NEWS & EVENTS

3rd Emerging Scientist Workshop of the SEBBM

The Spanish Society for Biochemistry and Molecular Biology organizes the 3rd Emerging Scientist Workshop, to be held in Santander (10-13 September 2018). This Workshop is devoted to those young and promising scientists at the stage of consolidating their careers as independent researchers, between their PhD and a permanent position. It aims to provide a platform from which emerging scientists can show new research lines or recent works and projects with the potential to be incorporated to Research Centers and Institutes. (more…)

The TubR–centromere complex adopts a double-ring segrosome structure in Type III partition systems

In prokaryotes, the centromere is a specialized segment of DNA that promotes the assembly of the segrosome upon binding of the Centromere Binding Protein (CBP). Here, we combine biochemical reconstruction and structural and biophysical analysis to bring light to the architecture of the segrosome complex in Type III partition systems.

Force determination in lateral magnetic tweezers combined with TIRF microscopy

Combining single-molecule techniques with fluorescence microscopy has attracted much interest because it allows the correlation of mechanical measurements with directly visualized DNA : protein interactions. In particular, its combination with total internal reflection fluorescence microscopy (TIRF) is advantageous because of the high signal-to-noise ratio this technique achieves.

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