The 2023 edition of the Macromolecular
Structures Department Workshop at the CNB-CSIC will focus on Advanced Biophysical Approaches to foster our knowledge on protein spectrometry and sequencing, nucleic acids nanostructures, mechanisms of molecular motors, nanopores, and mechanobiology.
Topics
Mass Photometry
Protein Sequencing
Optical & Magnetic Tweetzers
Atomic Force Microscopy
Fluorescence based methods
Program
9:20 – 9:30 Welcome
F. Moreno-Herrero. Main Organizer (CNB-CSIC)
Session 1: Advanced biophysical approaches based on microscopy, optical and magnetic tweezers
Session Chair: Fernando Moreno-Herrero (CNB-CSIC, Madrid)
9:30 – 10:15 Keynote TALK
Unravelling biomolecular structure, interactions and dynamics with mass photometry
Philipp Kukura (University of Oxford, UK)
10:15 – 10:35
Unveiling the contribution of lncRNA NIHCOLE to the repair of DNA breaks using Magnetic Tweezers and Fluorescence-correlated Optical Trapping
Sara de Bragança (CNB-CSIC, Madrid)
10:35 – 10:55
Comparative analysis of force generation by dynamin isoforms during membrane remodelling
Borja Ibarra (IMDEA-Nanociencia, Madrid, Spain)
10:55 – 11:15
Exploring DNA repair at the single-molecule level
Silvia Hormeño (CNB-CSIC, Madrid)
11.15 – 12.00 Coffee Break (sponsored by LumicksTM ) + Poster Sesion
Session 2: Advanced biophysical approaches based on nanopores
Session Chair: Sara de Bragança (CNB-CSIC, Madrid)
12:00 – 12:45 Keynote TALK
The timing of life at the nanoscale
Sonja Schmid (Wageningen University & Research, The Netherlands)
12:45 – 13:05
DNA nanotechnology to engineer advanced nanomaterials for biomedical applications
Silvia Hernandez-Ainsa (Institute of Nanoscience and Materials of Aragon, CSIC-University of Zaragoza, Zaragoza)
13:05 – 13:25
Analysis of single protein molecules with nanopores
David Rodriguez-Larrea (Instituto Biofisika, Bilbao)
13:25 – 13:45
Getting a grip on dynamic single molecules with correlative optical tweezers
Emma Verver (LUMICKS TM, The Netherlands)
13:45 – 15:00 Lunch + Posters Sesion
Session 3: Advanced biophysical approaches based on AFM
Session Chair: Mikel Marin Baquero (CNB-CSIC, Madrid)
15:00 – 15:45 Keynote TALK
Decoding mechanical fingerprints of cellular components in pathological conditions: a multiscale perspective
Andra Dumitru (CNIC, Spain)
15:45 – 16:05
Physical Virology with atomic force and fluorescence microscopies: exploring the biophysics of individual virus particles
Pedro J. de Pablo (UAM, Madrid)
16:05 – 16:25
How much force is needed to kill a single bacterium?
Cristina Flors (IMDEA-Nanociencia, Madrid)
16:25 – 16:45
Structural analysis of single-stranded RNA molecules using Atomic Force Microscopy
Eva M. Martin (CNB-CSIC, Madrid)
16:45 – 17:05
Nanorheology and Nanoindentation Revealed a Softening and an Increased Viscous Fluidity of Adherent Mammalian Cells upon Increasing the Frequency
Victor G. Gisbert (ICMM, Madrid)
17:05 – 17:25
Bridging the scales in DNA flexibility from nucleotides to single-molecule techniques via molecular simulations
Salvatore Assenza (UAM, Madrid)
17:25 Closing and farewell
Funding
