July 2018

July (2018). F. Moreno-Herrero and C.Lopez-Pasatrana visit Lumicks Headquarters in Amsterdam.

July (2018). We welcome M. Alvarez and B. Lago for a one-month stay in our lab.

 

June 2018

June (2018). A. Martin-Gonzalez wins the SPM Image Contest at Fuerzas y Tunel Conference, Jaca.

June (2018). A. Martin-Gonzalez talk and A. Marin-Gonzalez poster at Fuerzas y Tunel Conference, Jaca.

June (2018). J. Madariaga-Marcos talk (Best talk award!) and A. Marin-Gonzalez poster (Best poster award!) at SBE Conference, Castellon.

June (2018). J. Madariaga-Marcos talk and A. Martin-Gonzalez talk at 8th Workshop of Young Researchers in Nanoscience.

June (2018). We welcome Tung Le and Adam Jalal for a short visit to our lab.

 

May 2018

May (2018). Toseland´s and Moreno´s lab meeting in Madrid. First meeting of the i-Link Project funded by CSIC.

May (2018). New paper published in Nucleic Acids Research on TubR–centromere complex.

May (2018). S. Hormeño talk at the B-6 Department Seminars, CNB, Madrid.

 

3rd Emerging Scientist Workshop of the SEBBM

The Spanish Society for Biochemistry and Molecular Biology organizes the 3rd Emerging Scientist Workshop, to be held in Santander (10-13 September 2018).

This Workshop is devoted to those young and promising scientists at the stage of consolidating their careers as independent researchers, between their PhD and a permanent position. It aims to provide a platform from which emerging scientists can show new research lines or recent works and projects with the potential to be incorporated to Research Centers and Institutes.

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Supramolecular Assembly of Human Pulmonary Surfactant Protein SP-D

R. Arroyo, A. Martín-González, M. Echaide, A. Jain, W.H. Brondyk, J. Rosenbaum, F. Moreno-Herrero and J. Pérez-Gil

Journal of Molecular Biology (2018). Volume 430, Issue 10, 11 May 2018, Pages 1495-1509. Available online 4 April 2018. https://doi.org/10.1016/j.jmb.2018.03.027

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Pulmonary surfactant protein D (SP-D) is a glycoprotein from the collectin family that is a component of the lung surfactant system. It exhibits host defense and immune regulatory functions in addition to contributing to the homeostasis of the surfactant pool within the alveolar airspaces. It is known that the SP-D monomer forms trimers, which further associate into higher-order oligomers. However, the pathway and the interactions involved in the assembly of SP-D oligomers are not clearly understood. In the current study, a recombinant form of full-length human SP-D (rhSP-D) has been qualitatively and quantitatively studied by atomic force microscopy (AFM) and electrophoresis, with the aim to understand the conformational diversity and the determinants defining the oligomerization of the protein. The rhSP-D preparation studied is a mixture of trimers, hexamers, dodecamers and higher-order oligomeric species, with dodecamers accounting for more than 50% of the protein by mass. Similar structures were also found in hSP-D obtained from proteinosis patients, with the largest fuzzy-ball-like oligomers being more abundant in these samples. The proportion of dodecamer is increased under acidic conditions, accompanied by a conformational change into more compact configurations. Two hexamers appear to be the minimal necessary unit for dodecamer formation, with stabilization of the dodecamer occurring via non-covalent, ionic, and hydrophobic interactions between the individual N-terminal domains and the proximal area of the SP-D collagen stems.

 

Force determination in lateral magnetic tweezers combined with TIRF microscopy

Combining single-molecule techniques with fluorescence microscopy has attracted much interest because it allows the correlation of mechanical measurements with directly visualized DNA : protein interactions. In particular, its combination with total internal reflection fluorescence microscopy (TIRF) is advantageous because of the high signal-to-noise ratio this technique achieves.

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March 2018

March (2018). New paper accepted at Journal of Molecular Biology on Structural Characterization of Human Pulmonary Surfactant Protein SP-D by Atomic Force Microscopy.

March (2018). C. Lopez Pastrana talk at CNB entitled “Single-molecule biophysics meets hidden Markov models”.