Mechanical Properties and high resolution imaging of Nucleic Acids

We have investigated the mechanical properties of dsDNA and dsRNA using AFM, Magnetic Tweezers, and Optical Tweezers. GC-rich DNA shows in circular-dicroism experiments a signal compatible with A-form DNA. We investigated the mechanical properties and stability conditions of GC-rich DNA and compared these with a control DNA of equal GC/AT content under a variety of buffers and a wide range of forces. To do this study we used Magnetic Tweezers and AFM. 

IMDEA Nanociencia: B. Ibarra´s Group, R. Arias-Gonzalez´s Group

UAM. Science Faculty: J. Gomez-Herreros´s Group


S. Hormeno et al. Biophysical Journal 100, 1996-2006 (2011).

Mechanical Properties of High GoC-content DNA with A-type base-stacking


S. Hormeno et al. Biophysical Journal 100, 2006-2015 (2011).

Condensation prevails over B-A transition in the structure of DNA at low humidity”


S. E. Herrero-Galan et al. JACS 135(1), 122-131 (2013).

Mechanical identities of RNA and DNA double helices unveiled at the single-molecule level


Ares et al. Nanoscale 8,11818-11826 (2016).

High resolution atomic force microscopy of double-stranded RNA