DNA2 is an essential enzyme involved in DNA replication and repair in eukaryotes. In a search for homologues of this protein, we identified and characterised Geobacillus stearothermophilus Bad, a bacterial DNA helicase–nuclease with similarity to human DNA2.Continue Reading
A-tracts are A:T rich DNA sequences that exhibit unique structural and mechanical properties associated with several functions in vivo. The crystallographic structure of A-tracts has been well characterized. However, the mechanical properties of these sequences is controversial and their response to force remains unexploredContinue Reading
Surfactant Protein D (SP-D) is a collectin protein that participates in the innate immune defense of the lungs. SP-D mediates the clearance of invading microorganisms by opsonization, aggregation or direct killing, which are lately removed by macrophages.
SP-D is found as a mixture of trimers, hexamers, dodecamers and higher order oligomers, “fuzzy balls”. However, it is unknown whether there are differences between these oligomeric forms in functions, activity or potency.
Surfactant protein D (SP-D) is a C-type lectin that participates in the innate immune defense of lungs. It binds pathogens through its carbohydrate recognition domain in a calcium-dependent manner. Human surfactant protein D (hSP-D) has been routinely obtained from bronchoalveolar lavage of patients suffering from pulmonary alveolar proteinosis (PAP) and from amniotic fluid (AF). As a consequence of the disease, hSP-D obtained from PAP is found in higher amounts and is mainly composed of higher order oligomeric formsContinue Reading
The mechanical properties of double-stranded RNA (dsRNA) are involved in many of its biological functions and are relevant for future nanotechnology applications. DsRNA must tightly bend to fit inside viral capsids or deform upon the interaction with proteins that regulate gene silencing or the immune response against viral attacks. However, the question of how the nucleotide sequence affects the global mechanical properties of dsRNA has so far remained largely unexplored. Here, we have employed stateof-the-art atomistic molecular dynamics simulations to unveil the mechanical response of different RNA duplexes to an external forceContinue Reading
Fernando Moreno-Herrero together with Eduardo Oliver (CNIC), were responsible for the organization of “The 42nd Congress of the Spanish Biochemical and Molecular Biology Society”, that was held in Madrid on 16-19th July 2019, and count on the presence and support of the Minister of science, Innovation and Universities Pedro Duque during the Opening Ceremony.Continue Reading
Bacillus subtilis ParB forms multimeric networks involving non-specific DNA binding leading to DNA condensation. Previously, we found that an excess of the free C-terminal domain (CTD) of ParB impeded DNA condensation or promoted decondensation of pre-assembled networks (Fisher et al., 2017). However, interpretation of the molecular basis for this phenomenon was complicated by our inability to uncouple protein binding from DNA condensation.Continue Reading
Natural transformation contributes to acquisition and spread of antibiotic resistance and pathogenicity traits.We show that Bacillus subtilis chromosomal transformation decreased logarithmically with increased sequence divergence up to 15% in wild type (wt) cells or in cells lacking MutS2 or mismatch repair proteins (MutL, MutS or both).Continue Reading
February (2019). We welcome Mikel Marín Baquero to the lab, to carry out his PhD in AFM and fluorescence.
February (2019). New paper published in Physical Review Letters on the DNA Crookedness Regulates DNA Mechanical Properties at Short Length Scales.
February (2019). Nature Reviews Physics Highlight our on DNA crookedness and its relation with stretching flexibility. LINK
February (2019). Fernando attends the Spanish Mechano-Biology Network Meeting in Zaragoza and gives a talk.
February (2019). Clara, Silvia, Carolina y Alejandro participate on 11 Feb events:
International Day of Woman and Girls Science.
Clara, Silvia and Carolina carries out some experiments with milk and colorants here in the CNB.
Clara talks about unrolling DNA at the IES Severo Ochoa.
Alejandro talks about four famous female scientics and performs experiments to young students at the the social and cultural center La Casa Encendida in the center of Madrid.
February (2019) C. Carrasco gives a talk at the Mechanobiology Seminars at CNIC, Madrid.
February (2019). Julene Madriaga defends her PhD Thesis: Magnetic Tweezers and Fluorescence to study DNA – protein interactions.
Sequence-dependent DNA conformation and flexibility play a fundamental role in the specificity of DNA-protein interactions. Here we quantify the DNA crookedness: a sequence-dependent deformation of DNA that consists of periodic bends of the base pair centers chain.Continue Reading